Isolation and determination of maltooligosaccharides
نویسنده
چکیده
Maltooligosaccharides, up to maltooctaose, were converted to the corresponding 4-nitrophenylhydrazones and in this form effectively resolved by paper chromatography. A method of determination of maltooligosaccharides in mixtures was elaborated which includes elution of the separated 4-nitrophenylhydrazones from the paper and their spectrophotometric measurement. The analytical procedure was employed for assays of amylolytic activities in urine of healthy persons and patients suffering from acute pancreatitis. Isolation of [U-C]maltooligosaccharides from a [U-C]starch hydrolysate via 4-nitrophenylhydrazones is also described.
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